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KMID : 0903520050480030212
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2005 Volume.48 No. 3 p.212 ~ p.216
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Inhibitory Effect of Thapsigargin on the Activities of H+-ATPases in Tomato Roots
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Cho Kwang-Hyun
Kim Young-Kee
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Abstract
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Thapsigargin is a specific antagonist of SR/ER-type Ca2+-ATPase in animal tissue, and it was used to characterize the microsomal ATPases prepared from the roots of tomato. When 10 μM thapsigargin was added, it inhibited the microsomal ATPase activity by 30%. The thapsigargin-induced inhibition was dose-dependent. Since the activity of Ca2+-ATPase is very low in the roots of tomato tissue, it is possible that thapsigargin inhibits the activities of major H+-ATPases located in plasma and vacuolar membranes. The inhibitory effect of thapsigargin was reduced when the vacuolar H+- ATPase activity was inhibited by NO3 ?. However, the effect of thapsigargin was not observed on the H+-ATPase activity located in the plasma membrane. These results suggest that thapsigargin inhibits the vacuolar H+-ATPase activity in the roots of tomato.
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KEYWORD
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H+-ATPase, Nitrate, Thapsigargin, Tomato roots, Vanadate
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